Elicitation and Enhancement of Phenolics Synthesis with Zinc Oxide Nanoparticles and LED Light in Lilium candidum L. Cultures In Vitro

In this study, we identified and determined the content of phenolic compounds in Lilium candidum adventitious bulbs formed vitro. HPLC analysis revealed presence four acids: chlorogenic, caffeic, p-coumaric, ferulic acid. Phenolic acid was assessed vitro on media supplemented with zinc oxide nanoparticles (ZnO NPs at 25, 50, 75 mg/L) under fluorescent light (FL) or darkness (D). The second experiment analyzed effects light-emitting diodes (LEDs) variable spectra formation their contents acids. Spectral compositions red (R; 100%), blue (B; (RB; 70% 30%, respectively), a mix RB green (RBG) equal proportions (50%), white (WLED, 33.3% warm, neutral, cool light, proportionately) were used study. FL D conditions as controls for spectra. Bulbs grown soil served control samples. most abundant p-coumaric Treatment LED spectra, i.e., RB, RBG, WLED, B, translated into highest concentration compared other treatments. Moreover, all including those ZnO contained more than bulbscales soil. On hand, accumulated about two to three times higher amounts chlorogenic We also found that levels examined phenolics decreased FL, R, conditions, while bulblets showed content. use increased caffeic darkness.